The TNFAIP3 Monoclonal Antibody (CAB19128) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. RRID AB_2862621 Gene ID 7128 Swiss Prot Synonym A20; AISBL; AIFBL1; OTUD7C; TNFA1P2; TNFAIP3
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TNFAIP3 Monoclonal Antibody
SKU:
CAB19128
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0355
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
A20, AISBL, AIFBL1, OTUD7C, TNFA1P2, TNFAIP3
Positive Sample:
Jurkat, HeLa
Cellular Localization:
Cytoplasm, Lysosome, Nucleus.
Calculated MW:
90kDa
Observed MW:
80-90kDa
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. RRID AB_2862621 Gene ID 7128 Swiss Prot Synonym A20; AISBL; AIFBL1; OTUD7C; TNFA1P2; TNFAIP3
Purification Method:
Affinity purification
Gene ID:
7128
RRID:
AB_2862621
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TNFAIP3 Rabbit mAb (CAB19128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TNFAIP3 Rabbit mAb (CAB19128) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
