ZDHHC1 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
ZDHHC1 Antibody (PACO48022)
SKU:
PACO48022
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from human ZDHC1 AA range: 81-131
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q8WTX9
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
WB 1:1000-1:3000, IHC 1:50-1:200, IF 1:20-1:100
Synonyms:
ZDHHC1, C16orf1, ZNF377, Palmitoyltransferase ZDHHC1, DHHC domain-containing cysteine-rich protein 1, Zinc finger DHHC domain-containing protein 1, DHHC-1, Zinc finger protein 377
Target Names:
ZDHHC1
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification:
Antigen affinity purification
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western Blot Positive WB detected in: A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), U251 whole cell lysate(20µg), 293 whole cell lysate(20µg), Mouse Brain whole cell lysate(20µg),Mouse Spleen tissue lysate(20µg) All lanes: ZDHHC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 55 kDa Observed band size: 55 kDa Exposure time: 10s
IHC image of PACO48022 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Immunofluorescence staining of U251 cell with PACO48022 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with PACO48022 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
