The 4-HNE ELISA Kit is designed for the quantitative detection of 4-Hydroxynonenal (4-HNE) levels in various biological samples. 4-HNE is a critical biomarker of oxidative stress and lipid peroxidation, involved in a wide array of cellular activities, including cell proliferation, differentiation, and apoptosis. It plays a significant role in the body's response to oxidative stress and has been implicated in diverse diseases, including cardiac disorders, neurodegenerative diseases, and cancer. Accurate measurement of 4-HNE is essential for understanding its involvement in disease mechanisms and for developing targeted therapeutic strategies.
Assay Genie's 4-HNE ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's 4-HNE ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
4-HNE (4-Hydroxynonenal) ELISA Kit
SKU:
AEES00007
Target:
4-HNE (4-Hydroxynonenal)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
97-115
93-107
Average (%)
103
105
101
1:4
Range (%)
91-103
95-106
93-107
Average (%)
97
100
99
1:8
Range (%)
86-102
90-102
99-109
Average (%)
93
97
104
1:16
Range (%)
83-96
91-106
97-111
Average (%)
90
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
91-107
99
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.9
5.4
18.0
1.9
5.8
19.1
Standard deviation
0.1
0.2
0.6
0.1
0.3
0.6
C V (%)
5.26
3.7
3.33
5.26
5.17
3.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed.
