The OPLAH Antibody (CAB12032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.5-Oxoprolinase is a key enzyme in the gamma-glutamyl cycle, playing a crucial role in maintaining cellular redox homeostasis and protecting cells from oxidative damage. Dysregulation of this enzyme has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
OPLAH Antibody
SKU:
CAB12032
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OPLA, OPLAHD, 5-Opase, OPLAH
Positive Sample:
K-562, Mouse brain, Mouse kidney, Mouse liver
Cellular Localization:
Cytosol.
Calculated MW:
137kDa
Observed MW:
134kDa
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Purification Method
Affinity purification
Gene ID
26873
RRID
AB_2758943
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using OPLAH Rabbit pAb (CAB12032) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
