The A-CoA (Acetyl Coenzyme A) ELISA Kit is designed for the quantification of Acetyl Coenzyme A levels in various biological samples. Acetyl Coenzyme A plays a central role in the metabolism of carbohydrates and fats, acting as a key intermediate in numerous biosynthetic reactions. It is crucial for producing energy in cells, making it a critical component in metabolic functioning. Accurately quantifying levels of Acetyl Coenzyme A is essential for understanding its role in metabolic disorders and for developing targeted therapeutics.
Assay Genie's A-CoA ELISA Kit offers unbeatable sensitivity and specificity, believing in the provision of reliable and reproducible results. Made under strict quality control standards, this kit delivers robust performance and is user-friendly, being an optimal choice in both research and clinical settings. Trust Assay Genie's A-CoA ELISA Kit for precise and reliable quantitation of this key metabolic biomarker in your experiments.
Product Name:
A-CoA (Acetyl Coenzyme A) ELISA Kit
SKU:
AEES00006
Target:
A-CoA (Acetyl Coenzyme A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal A-CoA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal A-CoA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal A-CoA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal A-CoA. You can calculate the concentration of Universal A-CoA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
93-110
92-109
Average (%)
96
100
99
1:4
Range (%)
90-102
82-98
84-97
Average (%)
96
89
91
1:8
Range (%)
93-106
82-93
87-101
Average (%)
99
87
93
1:16
Range (%)
90-105
81-95
88-102
Average (%)
97
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.11
2.68
7.94
1.0
2.48
7.46
Standard deviation
0.08
0.12
0.4
0.07
0.11
0.27
C V (%)
7.21
4.48
5.04
7.0
4.44
3.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of A-CoA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes A-CoA in samples. No significant cross-reactivity or interference between A-CoA and analogues was observed.
