The ACADL Antibody (CAB1266) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and validated for use in Western blot applications. By binding to the AcadL protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component in studies related to metabolism, energy regulation, and mitochondrial function.AcadL, also known as acyl-CoA dehydrogenase long chain, plays a crucial role in breaking down long-chain fatty acids for energy production in the mitochondria.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACADL Antibody
SKU:
CAB1266
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LCAD, ACAD4, ACADL
Positive Sample:
SH-SY5Y, Mouse liver, Mouse kidney, Mouse heart, Rat spinal cord
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
48kDa
Observed MW:
47kDa
The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia.
Purification Method
Affinity purification
Gene ID
33
RRID
AB_2759506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACADL Rabbit pAb (CAB1266) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using ACADL Rabbit pAb (CAB1266) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using ACADL Rabbit pAb (CAB1266) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
