The ACAP2 Antibody (CAB12127) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples. By binding to the ACAP2 protein, this antibody enables detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and molecular biology.ACAP2, also known as Arf-GAP with coiled-coil, Ankyrin repeat and PH domains 2, plays a crucial role in regulating vesicle trafficking and membrane remodeling within cells.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ACAP2 Antibody
SKU:
CAB12127
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables GTPase activator activity. Acts upstream of or within actin filament-based process. Located in ruffle.
Purification Method
Affinity purification
Gene ID
23527
RRID
AB_2759016
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACAP2 Rabbit pAb (CAB12127) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb (CAB12127) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
