The ACER2 Polyclonal Antibody (PACO22136) is a crucial tool for researchers studying the role of ACER2 in various biological processes. ACER2 is a key enzyme involved in sphingolipid metabolism, specifically in the regulation of ceramide levels. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.ACER2 is known for its involvement in cellular signaling pathways related to cell survival, apoptosis, and inflammation. By targeting ACER2 with this antibody, researchers can effectively detect and analyze ACER2 expression in different cell types, allowing for deeper insights into its functions in physiological and pathological conditions.
This makes the antibody an invaluable tool for studies in lipid metabolism, cell signaling, and potential therapeutic interventions related to ACER2 dysregulation.Overall, the ACER2 Polyclonal Antibody (PACO22136) provides a reliable means of investigating the biological roles of ACER2 in various research areas, including cancer biology, neurodegenerative diseases, and metabolic disorders. By understanding the mechanisms underlying ACER2 activity, researchers can work towards developing targeted therapies and interventions aimed at modulating sphingolipid metabolism for improved health outcomes.
Antibody Name:
ACER2 Antibody (PACO22136)
Antibody SKU:
PACO22136
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human ASAH3L.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COS7 cells, using ASAH3L antibody.
Background:
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)-ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)-ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.
ACER2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. Belongs to the alkaline ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - sphingolipid; EC 3.5.1.23; HydrolaseChromosomal Location of Human Ortholog: 9p22.1Cellular Component: Golgi membrane; Golgi apparatus; integral to Golgi membraneMolecular Function: ceramidase activityBiological Process: caspase activation; response to retinoic acid; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; negative regulation of cell-matrix adhesion; ceramide metabolic process; negative regulation of cell adhesion mediated by integrin
UniProt Protein Details:
NCBI Summary:
The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]
