The Acetyl-Histone H3-K27 Antibody (CAB7253) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ChIP-seq, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H3-K27 Antibody
SKU:
CAB7253
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPChIPChIP-seqELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
IP
0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2767797
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at 1:2000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at 1:2000 dilution. C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human spleen using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (CAB7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells treated with TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, CAB7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells treated with TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, CAB7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (CAB7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.
