The Acetyl-Histone H3-K9/K14/K18/K23/K27 Polyclonal Antibody (CAB21299) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets acetylated histone H3 at multiple lysine residues (K9/14/18/23/27), allowing for the detection and analysis of these modifications in various biological samples.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to acetylated histone H3 at K9/14/18/23/27, enabling researchers to study the impact of histone acetylation on gene transcription, DNA replication, and other chromatin-related processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
HeLa treated with TSA, NIH/3T3 treated with TSA, C6 treated with TSA
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb (CAB21299) at 1:1000 dilution. HeLa,NIH/3T3 and C2C12 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
