The ACOT2 Antibody (CAB14390) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for Western blot applications. By targeting the ACOT2 protein, researchers can effectively detect and analyze ACOT2 expression in various cell types, making it an ideal tool for studies in metabolism and energy regulation.ACOT2 plays a crucial role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA esters, which are essential intermediates in fatty acid metabolism.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACOT2 Antibody
SKU:
CAB14390
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MTE1, PTE2, CTE1A, PTE2A, CTE-IA, ZAP128, ACOT2
Positive Sample:
293T, LO2, U-87MG, Mouse kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
53kDa
Observed MW:
50kDa
This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10965
RRID
AB_2761259
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACOT2 Rabbit pAb (CAB14390) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human appendix using ACOT2 Rabbit pAb (CAB14390) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
