The ADAMTS5 Antibody (CAB2836) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has high reactivity with human samples, making it ideal for Western blot applications.ADAMTS5 plays a crucial role in tissue remodeling and degradation of aggrecan, a key component of cartilage. Dysregulation of ADAMTS5 has been linked to cartilage degradation and joint inflammation in osteoarthritis. By targeting ADAMTS5, researchers can investigate its role in disease progression and potentially develop targeted therapies for osteoarthritis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ADAMTS5 Antibody
SKU:
CAB2836
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis.
Purification Method
Affinity purification
Gene ID
11096
RRID
AB_2764666
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ADAMTS5 Rabbit pAb (CAB2836) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): THP-1. Exposure time: 20s.
