The ADH4 Antibody (CAB5454) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human ADH4 samples and has been validated for use in Western blot applications. By targeting the ADH4 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in biochemistry and pharmacology.ADH4 is known for its ability to metabolize alcohol, playing a key role in the breakdown of ethanol in the liver.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
ADH4 Antibody
SKU:
CAB5454
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ADH-2, HEL-S-4, ADH4
Positive Sample:
Mouse liver
Cellular Localization:
Cytoplasm.
Calculated MW:
40kDa
Observed MW:
45kDa
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
Purification Method
Affinity purification
Gene ID
127
RRID
AB_2766255
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse liver, using ADH4 Rabbit pAb (CAB5454) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
