The AGER Antibody (CAB13264) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blotting.AGER, also known as advanced glycosylation end product-specific receptor, plays a key role in mediating cellular responses to damage caused by high levels of blood sugar. Its involvement in inflammation and tissue damage makes it a promising target for research in diabetes, cardiovascular diseases, and other conditions related to glycation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AGER Antibody
SKU:
CAB13264
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAGE, sRAGE, SCARJ1, AGER
Positive Sample:
Rat lung
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
43kDa
Observed MW:
45kDa/55kDa
The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847).
Purification Method
Affinity purification
Gene ID
177
RRID
AB_2760117
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using AGER Rabbit pAb (CAB13264) at 1:5000/1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
