The Angiotensinogen Monoclonal Antibody (CAB11689) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting angiotensinogen in human samples, making it an invaluable tool for studying the role of this protein in various physiological processes.Angiotensinogen is a precursor protein that plays a central role in the production of angiotensin peptides involved in blood pressure regulation, kidney function, and cardiovascular health. Dysregulation of the renin-angiotensin system is associated with various cardiovascular diseases, making angiotensinogen a target of interest for researchers studying hypertension, heart disease, and kidney disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Angiotensinogen Monoclonal Antibody
SKU:
CAB11689
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0677
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANHU, hFLT1, SERPINA8, Angiotensinogen
Positive Sample:
Mouse liver, Human plasma, Rat plasma
Cellular Localization:
Secreted.
Calculated MW:
53kDa
Observed MW:
52kDa
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
Purification Method
Affinity purification
Gene ID
183
RRID
AB_2861630
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse liver, using Angiotensinogen Rabbit mAb (CAB11689) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using Angiotensinogen Rabbit mAb (CAB11689) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
