The ALG2 Antibody (CAB7843) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the ALG2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for research in cell biology and molecular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ALG2 Antibody
SKU:
CAB7843
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDG1I, CDGIi, CMS14, NET38, CMSTA3, hALPG2, ALG2
Positive Sample:
MCF7, Mouse brain
Cellular Localization:
Membrane, Single-Pass Membrane Protein.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
85365
RRID
AB_2768334
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ALG2 Rabbit pAb (CAB7843) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 90s.
