The ALKBH1 Antibody (CAB14079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a variety of research applications, including Western blotting and immunohistochemistry.ALKBH1, a member of the AlkB family of proteins, plays a crucial role in maintaining genome stability by repairing alkylated DNA and oxidatively damaged RNA. Dysregulation of ALKBH1 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ALKBH1 Antibody
SKU:
CAB14079
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ABH, ABH1, alkB, hABH, ALKBH, ALKBH1
Positive Sample:
293T
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.
Purification Method
Affinity purification
Gene ID
8846
RRID
AB_2760934
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using ALKBH1 Rabbit pAb (CAB14079) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using ALKBH1 Rabbit pAb (CAB14079). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
