The ALKBH1 Antibody (CAB14079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. RRID AB_2760934 Gene ID 8846 Swiss Prot Synonym ABH; ABH1; alkB; hABH; ALKBH; ALKBH1
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ALKBH1 Antibody
SKU:
CAB14079
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ABH, ABH1, alkB, hABH, ALKBH, ALKBH1
Positive Sample:
293T
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. RRID AB_2760934 Gene ID 8846 Swiss Prot Synonym ABH; ABH1; alkB; hABH; ALKBH; ALKBH1
Purification Method:
Affinity purification
Gene ID:
8846
RRID:
AB_2760934
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using ALKBH1 Rabbit pAb (CAB14079) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using ALKBH1 Rabbit pAb (CAB14079). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
