Alpha-Mannosidase Activity Assay Kit (BA0014)

SKU:

BA0014

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Biological Samples Plasma, Serum, Tissue, Culture Media

Research Area:

Plant & Environmental Stress

Enzyme Activity

Description

Description

alpha-Mannosidase Activity Assay Kit - Information

Assay Genie's non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Applications

For quantitative determination of alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.

alpha-Mannosidase Activity Assay Kit - Key Features

Fast and sensitive. Linear detection range (10 sample): 1 to 250 U/L for a 10 minute reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.

alpha-Mannosidase Activity Assay Kit - Data Sheet
Kit Includes	Substrate Buffer: 10 mL Stop Reagent: 12 mL Standard: 1 mL 12.5 mM Nitrophenol
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader.
Method of Detection	OD405nm
Detection Limit	1 U/L
Samples	Biological samples e.g. plasma, serum, tissue and culture media.
Species	All
Protocol Length	10 min
Size	100 tests
Storage	Store all components at 4°C upon receiving.
Shelf Life	1 U/L

More Details

alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes alpha-mannosidosis and leads to deterioration of the central nervous system in children.