Alpha-Mannosidase Activity Assay Kit (BA0014)
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alpha-Mannosidase Activity Assay Kit - InformationAssay Genie's non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
ApplicationsFor quantitative determination of alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.
alpha-Mannosidase Activity Assay Kit - Key Features
- Fast and sensitive. Linear detection range (10 sample): 1 to 250 U/L for a 10 minute reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.
alpha-Mannosidase Activity Assay Kit - Data Sheet
|Kit Includes||Substrate Buffer: 10 mL Stop Reagent: 12 mL Standard: 1 mL 12.5 mM Nitrophenol|
|Kit Requires||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader.|
|Method of Detection||OD405nm|
|Detection Limit||1 U/L|
|Samples||Biological samples e.g. plasma, serum, tissue and culture media.|
|Protocol Length||10 min|
|Storage||Store all components at 4°C upon receiving.|
|Shelf Life||1 U/L|