This kit is comprised by HRP conjugate, other reagents and ELISA Microtiter plate pre-coated with Alpha-Toxin gene of clostridium perfringens (CP-α) antigen. Apply the principle of enzyme-linked immunoassay (ELISA) to detect CP-α-Ab in serum of goat and sheep. During the experiment, add control and samples into the ELISA Microtiter plate. If CP-α-Ab exist in the samples, it will be bound with the antigen on the ELISA Microtiter plate. Then wash the plate to remove unbound components, add the HRP conjugate to specifically bind with the compound of antibody and antigen on the microtiter plate. The unbound HRP Conjugate will be removed by washing. Substrate Reagent is added into the well, it will react with the enzyme and become blue. The color shade is positive correlation with CP-α-Ab levels in the samples. At last, end the reaction by adding stop solution to produce a yellow product. Measure the absorbance value of each well by using a Microplate Reader with 450 nm wavelength, then we can judge whether CP-α-Ab exist in the sample.
