The Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.
This antibody is validated for use in WB, IF/ICC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Alpha-tubulin (ubiquitous) chain Rabbit pAb
SKU:
CABC025
Size:
100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
Synonyms:
K-ALPHA-1, Alpha-tubulin (ubiquitous) chain
Positive Sample:
HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain , Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
50kDa
Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.
Purification Method
Affinity purification
Gene ID
10376
RRID
AB_2768344
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC025) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
