The ANAPC5 Antibody (CAB7109) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By targeting the ANAPC5 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component in cell biology and cancer research studies.As a crucial component of the APC/C complex, ANAPC5 plays a vital role in promoting the degradation of specific cell cycle proteins during mitosis and maintaining genomic stability.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ANAPC5 Antibody
SKU:
CAB7109
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Purification Method
Affinity purification
Gene ID
51433
RRID
AB_2767664
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] ANAPC5 Rabbit pAb (CAB7109) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and ANAPC5 knockout (KO) 293T cells, using [KO Validated] ANAPC5 Rabbit pAb (CAB7109) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] ANAPC5 Rabbit pAb (CAB7109) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg ANAPC5 antibody (CAB7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (CAB7109) at a dilution of 1:1000.
