The ANXA6 Antibody (CAB5390) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Annexin A6, this antibody enables the accurate detection and analysis of the protein in a variety of cell types, making it well-suited for investigations in cell biology and cancer research.Annexin A6 is a multifunctional protein that plays a key role in intracellular membrane trafficking and organization. Studies have shown its involvement in processes such as endocytosis, exocytosis, and receptor signaling, highlighting its importance in cellular function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ANXA6 Antibody
SKU:
CAB5390
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:7000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p68, p70, ANX6, CBP68, CPB-II, Annexin A6/Annexin VI
Positive Sample:
Jurkat, HeLa, C2C12, PC-12, C6
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
76kDa
Observed MW:
72kDa
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
309
RRID
AB_2766199
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Annexin A6/Annexin VI Rabbit pAb (CAB5390) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
