The α-Tubulin Mouse mAb (CABC012) is a high-quality antibody developed for reliable detection and analysis of target proteins. Alpha-tubulin forms the α/β-tubulin heterodimer with beta-tubulin, constituting the major building block of microtubules that perform essential and diverse cytoskeletal functions in eukaryotes. This gene encodes a highly conserved alpha-tubulin homolog, and precise regulation of tubulin expression is critical for proper microtubule dynamics across cell types.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
α-Tubulin Mouse mAb
SKU:
CABC012
Size:
20μL, 50μL, 100μL, 200μL
Reactivity:
Human, Mouse, Rat
Clone Number:
AMC0479
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:5000 - 1:20000
IHC-P
1:500 - 1:2000
IF/ICC
1:200 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALS22, TUBA1, H2-ALPHA, α-Tubulin
Positive Sample:
HeLa, NIH/3T3, RAW 264.7, C2C12, C6, H9c2(2-1), COS-7
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
55kDa
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7277
RRID
AB_2768341
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using α-Tubulin Mouse mAb (CABC012) at 1:20000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using α-Tubulin Mouse mAb (CABC012) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using α-Tubulin Mouse mAb (CABC012) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (CABC012, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C2C12 cells using α-Tubulin Mouse mAb (CABC012, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using α-Tubulin Mouse mAb (CABC012, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
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