The Anti B4GALT1 Chimeric Recombinant Rabbit Monoclonal Antibody (HDAB0260) is a cutting-edge tool designed for research involving B4GALT1, a crucial enzyme involved in glycosylation processes. This antibody, produced using advanced chimeric recombinant technology, boasts high specificity and sensitivity for detecting B4GALT1 in various samples, particularly human tissues.B4GALT1 plays a key role in the modification of glycoproteins and glycolipids, impacting cell adhesion, signaling, and immune response regulation. Dysregulation of B4GALT1 has been implicated in various diseases, including cancer and immune disorders, making it a valuable target for research in these fields.
The Anti B4GALT1 Chimeric Recombinant Rabbit Monoclonal Antibody enables precise detection and analysis of B4GALT1 levels, allowing for in-depth studies in cell biology, immunology, and disease mechanisms.With its superior performance in techniques like Western blotting, immunofluorescence, and flow cytometry, this antibody provides researchers with a reliable tool for investigating the function and significance of B4GALT1 in health and disease. Its versatility and high-quality performance make it an indispensable asset for labs conducting cutting-edge research in molecular biology and biomedicine.
SKU:
HDAB0260
Size:
100 µg
Clonality:
Monoclonal
Clone:
DMC392
Synonyms:
GGTB2, Beta4Gal-T1, b4Gal-T1, Nal synthase
Applications:
Flow Cyt
Recommended Dilution:
Flow Cyt 1:100
Host Species:
Rabbit
Isotype:
Rabbit/Human Fc chimeric IgG1
Reactivity:
Human
Purification Method:
Purified from cell culture supernatant by affinity chromatography
Formulation:
Powder
Buffer:
1XPBS
Storage:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
Usage:
Research use only
Background:
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose D-glucose UDP lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
