The β-Actin Mouse mAb (CABC004) is a high-quality antibody developed for reliable detection and analysis of target proteins. Beta-actin is a major constituent of the contractile apparatus and one of the two ubiquitously expressed nonmuscle cytoskeletal actins, playing essential roles in cell motility, structural integrity, and intercellular signaling. Mutations in this gene cause Baraitser-Winter syndrome 1, characterized by intellectual disability and a distinctive facial appearance.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
β-Actin Mouse mAb
SKU:
CABC004
Size:
20μL, 50μL, 100μL, 200μL
Reactivity:
Human, Mouse, Rat
Clone Number:
AMC0001
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:10000-1:100000
IHC-P
1:1000 - 1:10000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRWS1, PS1TP5BP1, β-Actin
Positive Sample:
293T, HeLa, A-549, NIH3T3, Mouse brain, Rat testis, C6
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Purification Method
Affinity purification
Gene ID
60
RRID
AB_2737399
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (CABC004) at 1:2000- 1:640000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using β-Actin Mouse mAb (CABC004) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using β-Actin Mouse mAb (CABC004) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using β-Actin Mouse mAb (CABC004) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of U-2 OS cells using β-Actin Mouse mAb (CABC004, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (CABS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
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