The β-Tubulin Mouse mAb (CABC021) is a high-quality antibody developed for reliable detection and analysis of target proteins. Beta-tubulin forms obligate heterodimers with alpha-tubulin as a core structural component of microtubules, essential for cell division, intracellular transport, and cell architecture maintenance. Mutations in this gene cause cortical dysplasia complex with brain malformations, reflecting its critical role in neuronal development.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii samples.
Product Name:
β-Tubulin Mouse mAb
SKU:
CABC021
Size:
100μL
Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii
Clone Number:
AMC0498
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:5000 - 1:10000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
IP
0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
SH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
Purification Method
Affinity purification
Gene ID
203068
RRID
AB_2773004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using β-Tubulin Mouse mAb (CABC021) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using β-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (CABC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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