The Brachyury Monoclonal Antibody (CAB5078) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, shows high reactivity with human samples and has been validated for use in various applications, including Western blotting.Brachyury is known for its role in notochord development and is often overexpressed in several cancer types, making it a promising target for cancer research. By detecting and analyzing Brachyury protein expression in different cell types, this antibody is a valuable resource for studies in developmental biology and oncology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Brachyury Monoclonal Antibody
SKU:
CAB5078
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1983
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T, TFT, SAVA, Brachyury
Positive Sample:
NCI-H460, Mouse brain, Mouse thymus, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
49kDa
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.
Purification Method
Affinity purification
Gene ID
6862
RRID
AB_2863435
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NCI-H460 cells, using Brachyury Rabbit mAb (CAB5078) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Brachyury Rabbit mAb (CAB5078) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
