The Calsequestrin 1 Monoclonal Antibody (CAB19640) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. RRID Gene ID 844 Swiss Prot Synonym CASQ; CSQ1; PDIB1; VMCQA; Calsequestrin 1
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Calsequestrin 1 Monoclonal Antibody
SKU:
CAB19640
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2209
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CASQ, CSQ1, PDIB1, VMCQA, Calsequestrin 1
Positive Sample:
Mouse skeletal muscle, Rat heart
Cellular Localization:
Endoplasmic Reticulum, Mitochondrial Matrix.
Calculated MW:
45kDa
Observed MW:
55kDa
This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. RRID Gene ID 844 Swiss Prot Synonym CASQ; CSQ1; PDIB1; VMCQA; Calsequestrin 1
Purification Method:
Affinity purification
Gene ID:
844
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat heart, using Calsequestrin 1 Rabbit mAb (CAB19640) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse skeletal muscle, using Calsequestrin 1 Rabbit mAb (CAB19640) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded Rat skeletal muscle using Calsequestrin 1 Rabbit mAb (CAB19640) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
