The CaMKI Monoclonal Antibody (CAB3529) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples and has been successfully validated for use in Western blot applications.By binding to the CAMKII protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for investigations in neuroscience, cell biology, and cardiovascular research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CaMKI Monoclonal Antibody
SKU:
CAB3529
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2030
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAMKI, CaMKI
Positive Sample:
Hep G2, Mouse lung, Mouse liver, Rat lung
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
41kDa
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Purification Method
Affinity purification
Gene ID
8536
RRID
AB_2863079
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CaMKI Rabbit mAb (CAB3529) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
