The CD48 Monoclonal Antibody (CAB4084) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the CD48 protein, it enables precise detection and analysis in a wide range of cell types, making it an ideal choice for studies within the fields of immunology and cancer research.CD48, also known as the signaling lymphocytic activation molecule (SLAM), plays a vital role in modulating immune responses and interactions between immune cells.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CD48 Monoclonal Antibody
SKU:
CAB4084
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC53030
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
962
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD48 Rabbit mAb (CAB4084) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Flow cytometry: 1X10^6 K-562 cells (negative control,left) and Daudi cells (right) were surface-stained with CD48 Rabbit mAb(CAB4084, 10 μg/mL orange line) or ABflo® 647 Rabbit IgG isotype control(AC042, 10 μg/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).
