The Cdc25C Monoclonal Antibody (CAB12234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the CDC25C protein, researchers can analyze and detect its expression in various cell types, making it a crucial reagent for studies in cancer biology and cell cycle regulation.CDC25C is essential for the progression of the cell cycle, playing a vital role in ensuring accurate DNA replication and cell division.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cdc25C Monoclonal Antibody
SKU:
CAB12234
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0622
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC25, PPP1R60, Cdc25C
Positive Sample:
HeLa, Mouse liver, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60-65kDa
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
Purification Method
Affinity purification
Gene ID
995
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cdc25C Rabbit mAb (CAB12234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using Cdc25C Rabbit mAb (CAB12234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
