The Cdc25C Monoclonal Antibody (CAB12234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. RRID Gene ID 995 Swiss Prot Synonym CDC25; PPP1R60; Cdc25C
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cdc25C Monoclonal Antibody
SKU:
CAB12234
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0622
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC25, PPP1R60, Cdc25C
Positive Sample:
HeLa, Mouse liver, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60-65kDa
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. RRID Gene ID 995 Swiss Prot Synonym CDC25; PPP1R60; Cdc25C
Purification Method:
Affinity purification
Gene ID:
995
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cdc25C Rabbit mAb (CAB12234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using Cdc25C Rabbit mAb (CAB12234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
