The Cdx1 Monoclonal Antibody (CAB9546) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human CDX1 samples, making it ideal for use in applications such as Western blotting.CDX1 is known for its involvement in gut development and maintenance, as well as its potential role in gastrointestinal cancers.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cdx1 Monoclonal Antibody
SKU:
CAB9546
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1627
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MYVG YVLD KDSP VYPG PARP ASLG LGPQ AYGP PAPP PAPP QYPD FSSY SHVE PAPA PPTA WGAP FPAP KDDW AAAY GPGP AAPA ASPA SLAF GPPP DFSP
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HT-29, SGC-7901
Cellular Localization:
Nucleus.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity.
Purification Method
Affinity purification
Gene ID
1044
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cdx1 Rabbit mAb (CAB9546) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
