The CDX2 Monoclonal Antibody (CAB20222) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including immunohistochemistry and Western blotting.CDX2 plays a crucial role in regulating gene expression in the intestinal epithelium, influencing cell proliferation, differentiation, and maintenance of intestinal function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CDX2 Monoclonal Antibody
SKU:
CAB20222
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC3070
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDX3, CDX-3, CDX2/AS, X2
Positive Sample:
SW620, 293T, Mouse colon
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
38kDa
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
Purification Method
Affinity purification
Gene ID
1045
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb (CAB20222) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (CAB20222) at1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
