The CENPB Antibody (CAB16817) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is optimized for detection of CENPB in human samples, making it a valuable tool for immunohistochemistry and other research applications.CENPB is essential for centromere function, playing a critical role in chromosome segregation and cell division. Aberrant expression or dysfunction of CENPB has been linked to various diseases, including cancer and genetic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CENPB Antibody
SKU:
CAB16817
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CENPB
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Nuclear Body, Nucleoplasm, Nucleus.
Calculated MW:
65kDa
Observed MW:
72kDa
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Purification Method
Affinity purification
Gene ID
1059
RRID
AB_2768849
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CENPB Rabbit pAb (CAB16817) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5min.
