The CENPC Monoclonal Antibody (CAB3975) is a high-quality antibody developed for reliable detection and analysis of target proteins. Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CENPC Monoclonal Antibody
SKU:
CAB3975
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC2097
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MIF2, hcp-4, CENP-C, CENPC1, CENPC
Positive Sample:
HeLa, Jurkat
Cellular Localization:
Cytosol, Nuclear Body, Nucleoplasm.
Calculated MW:
107kDa
Observed MW:
107kDa
Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.
Purification Method
Affinity purification
Gene ID
1060
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CENPC Rabbit mAb (CAB3975) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb (CAB3975) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
