The Chromogranin B Monoclonal Antibody (CAB3517) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. This protein may serve as a precursor for regulatory peptides.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Chromogranin B Monoclonal Antibody
SKU:
CAB3517
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC2023
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCG1, Chromogranin B
Positive Sample:
293T, PC-12, BxPC-3, SH-SY5Y
Cellular Localization:
Endoplasmic Reticulum Lumen, Extracellular Space.
Calculated MW:
78 kDa
Observed MW:
110 kDa
This gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. This protein may serve as a precursor for regulatory peptides.
Purification Method
Affinity purification
Gene ID
1114
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Chromogranin B Rabbit mAb (CAB3517) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
