Creatine kinase B type (CKB) Monoclonal Antibody (CAB4907)
The Creatine kinase B type (CKB) Monoclonal Antibody (CAB4907) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Creatine kinase B type plays a key role in ATP regeneration through the conversion of creatine and ADP to phosphocreatine and ATP. Dysregulation of this enzyme has been implicated in various diseases such as heart failure, neurodegenerative disorders, and muscular dystrophy.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Creatine kinase B type (CKB) Monoclonal Antibody
SKU:
CAB4907
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1990
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCK, B-CK, CKBB, CPK-B, HEL-211, HEL-S-29, Creatine kinase B type (CKB)
Positive Sample:
HeLa, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Cytosol, Mitochondrion, Cell Membrane.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
Purification Method
Affinity purification
Gene ID
1152
RRID
AB_2863385
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Creatine kinase B type (CKB) Rabbit mAb (CAB4907) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Creatine kinase B type (CKB) Rabbit mAb (CAB4907) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
