The Cytokeratin 18 (KRT18) Monoclonal Antibody (CAB19778) is a high-quality antibody developed for reliable detection and analysis of target proteins. KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. RRID Gene ID 3875 Swiss Prot Synonym K18; CK-18; CYK18; Cytokeratin 18 (KRT18)
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cytokeratin 18 (KRT18) Monoclonal Antibody
SKU:
CAB19778
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2303
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K18, CK-18, CYK18, Cytokeratin 18 (KRT18)
Positive Sample:
HeLa, Hep G2, A-431
Cellular Localization:
Adherens Junction, Cytoplasm, Cytoskeleton, Cytosol, Extracellular Exosome, Microtubule Organizing Center, Nuclear Matrix, Nucleolus, Perinuclear Region Of Cytoplasm.
Calculated MW:
48kDa
Observed MW:
48kDa
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. RRID Gene ID 3875 Swiss Prot Synonym K18; CK-18; CYK18; Cytokeratin 18 (KRT18)
Purification Method:
Affinity purification
Gene ID:
3875
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 18 (KRT18) Rabbit mAb (CAB19778) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cytokeratin 18 (KRT18) Rabbit mAb (CAB19778) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Cytokeratin 18 (KRT18) Rabbit mAb (CAB19778) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver using Cytokeratin 18 (KRT18) Rabbit mAb (CAB19778) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
