The Cytokeratin 6 (KRT6) Monoclonal Antibody (CAB19827) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in immunohistochemistry and Western blot applications.Cytokeratin 6 is known to play a role in maintaining the structural integrity of epithelial tissues and is often upregulated in conditions such as wound healing and cancer. The CAB19827 Antibody binds specifically to cytokeratin 6, allowing for precise detection and analysis in various cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cytokeratin 6 (KRT6) Monoclonal Antibody
SKU:
CAB19827
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2352
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K6E, KRT6E, PPKNEFD, Cytokeratin 6 (KRT6)
Positive Sample:
5637, A-431
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
60kDa
Observed MW:
60kDa
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13.
Purification Method
Affinity purification
Gene ID
286887
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
