The D14 Polyclonal Antibody (CAB20661) is a valuable tool for researchers studying the D14 protein, a key player in plant hormone signaling pathways. This antibody, produced in rabbits, is highly specific for detecting D14 in plant samples and has been validated for use in various applications, including Western blotting. By binding to the D14 protein, this antibody enables precise detection and analysis in different plant tissues, making it an essential component for studies in plant biology and agriculture.D14 is a receptor protein that plays a crucial role in the perception and transduction of strigolactone signals, a class of plant hormones involved in diverse physiological processes, such as root development and response to environmental stress.
Understanding the function of D14 is essential for unraveling the complexity of plant hormone signaling pathways and developing strategies to improve crop yield and stress tolerance. Researchers studying plant growth and development will benefit from the use of the D14 Polyclonal Antibody to explore the intricate mechanisms underlying plant hormone responses.
Product Name:
D14 Polyclonal Antibody
SKU:
CAB20661
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Oryza sativa
Immunogen:
Recombinant protein of Oryza sativa D14.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
D14; D88; HTD2
Positive Sample:
leaves,Stems,Spikes
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, nucleus
Calculated MW:
34kDa
Observed MW:
36kDa
Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form.
Purification Method:
Affinity purification
Gene ID:
4331983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using D14 Rabbit pAb (CAB20661) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
