The DTX1 Antibody (CAB20499) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in a variety of applications, including Western blot analysis.DTX1, also known as deltex E3 ubiquitin ligase 1, plays a crucial role in regulating the Notch signaling pathway, which is involved in various cellular processes like cell differentiation, proliferation, and apoptosis. Dysregulation of the Notch pathway has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DTX1 Antibody
SKU:
CAB20499
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
hDx-1, RNF140, DTX1
Positive Sample:
Daudi
Cellular Localization:
Cytoplasm, Cytosol, Nuclear Body, Nucleoplasm.
Calculated MW:
67kDa
Observed MW:
67-72kDa
Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity.
Purification Method
Affinity purification
Gene ID
1840
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Daudi cells using DTX1 Rabbit pAb (CAB20499) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
