The DYNC1LI2 Antibody (CAB20592) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and reactivity with human samples, making it ideal for use in Western blot applications.DYNC1LI2 is a crucial component of the dynein complex, which plays a pivotal role in various cellular processes such as organelle transport, mitosis, and cell division. Dysfunction of DYNC1LI2 has been implicated in neurodegenerative diseases and developmental disorders, making it a promising target for investigation in these areas.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DYNC1LI2 Antibody
SKU:
CAB20592
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LIC2, DNCLI2, DYNC1LI2
Positive Sample:
Mouse testis, Mouse brain, Rat lung, Rat brain
Cellular Localization:
Centrosome, Cytosol, Late Endosome, Lysosome.
Calculated MW:
54kDa
Observed MW:
54kDa
Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al., 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.
Purification Method
Affinity purification
Gene ID
1783
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DYNC1LI2 Rabbit pAb (CAB20592) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DYNC1LI2 Rabbit pAb (CAB20592) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using DYNC1LI2 Rabbit pAb (CAB20592) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using DYNC1LI2 Rabbit pAb (CAB20592) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
