The EGR2 Monoclonal Antibody (CAB3219) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EGR2 Monoclonal Antibody
SKU:
CAB3219
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1932
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AT591, CMT1D, CMT4E, KROX20, EGR2
Positive Sample:
PC-3, SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
50kDa
Observed MW:
50kDa
The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1959
RRID
AB_2863028
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EGR2 Rabbit mAb (CAB3219) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg EGR2 antibody (CAB3219). Western blot was performed from the immunoprecipitate using EGR2 antibody (CAB3219) at a dilution of 1:500.
