The Fibronectin Monoclonal Antibody (CAB12977) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting fibronectin in human samples. Validated for use in Western blotting applications, this antibody enables the detection and analysis of fibronectin in a variety of cell types.Fibronectin plays a critical role in various biological processes, including wound healing, embryonic development, and tissue remodeling. Dysregulation of fibronectin expression has been linked to diseases such as cancer, fibrosis, and cardiovascular disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Fibronectin Monoclonal Antibody
SKU:
CAB12977
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC2692
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Purification Method
Affinity purification
Gene ID
2335
RRID
AB_2759824
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells, using Fibronectin Rabbit mAb (CAB12977) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Confocal imaging of HeLa cells using Fibronectin Rabbit mAb (CAB12977,dilution 1:50)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
