The FOXL2 Monoclonal Antibody (CAB3597) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is suitable for use in various applications such as immunofluorescence, immunohistochemistry, and Western blotting.FOXL2 is crucial for the development and maintenance of ovarian tissue and plays a vital role in the regulation of genes involved in ovarian function. Mutations in the FOXL2 gene have been linked to disorders such as blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES), as well as ovarian failure.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FOXL2 Monoclonal Antibody
SKU:
CAB3597
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2055
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BPES, PFRK, POF3, BPES1, PINTO, FOXL2
Positive Sample:
HeLa, K-562, Mouse testis, Rat testis, Rat ovary
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
50kDa
This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3.
Purification Method
Affinity purification
Gene ID
668
RRID
AB_2863098
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FOXL2 Rabbit mAb (CAB3597) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using FOXL2 Rabbit mAb (CAB3597) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
