The HCoV-HKU1 Nucleoprotein Antibody (CAB20612) is a high-quality antibody developed for reliable detection and analysis of target proteins. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-HKU1 samples.
Product Name:
HCoV-HKU1 Nucleoprotein Antibody
SKU:
CAB20612
Size:
100μL, 20μL
Reactivity:
HCoV-HKU1
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Human coronavirus (HCoV-HKU1) Nucleocapsid Protein (His Tag)
Calculated MW:
49kDa
Observed MW:
50-60kDa
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Purification Method
Affinity purification
Gene ID
3200423
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-HKU1) Nucleocapsid Protein (His Tag), using HCoV-HKU1 Nucleoprotein Rabbit pAb (CAB20612) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
