The HRP-conjugated β-Tubulin Mouse mAb (CABC030) is a high-quality antibody developed for reliable detection and analysis of target proteins. Beta-tubulin forms structural heterodimers with alpha-tubulin as a core component of microtubules, essential for mitosis, intracellular transport, and cell morphology. Its ubiquitous and consistent expression across eukaryotic cell types makes it a reliable loading control in western blotting experiments.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HRP-conjugated β-Tubulin Mouse mAb
SKU:
CABC030
Size:
100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
AMC0499
Conjugate:
HRP
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa, U-87MG, 293T, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
55kDa
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
Purification Method
Affinity purification
Gene ID
203068
RRID
AB_2769870
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 50% glycerol,pH7.3.
Western blot analysis of various lysates using HRP-conjugated β-Tubulin Mouse mAb (CABC030) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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