The HSPA4 Monoclonal Antibody (CAB19705) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, specifically targets the HSPA4 protein and is highly reactive with human samples. Validated for use in Western blot applications, the HSPA4 antibody enables precise detection and analysis of the protein in different cell types.HSPA4, a member of the heat shock protein family, plays a crucial role in cellular stress response and protein folding. Its involvement in maintaining protein homeostasis makes it a key player in regulating cell function and viability.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSPA4 Monoclonal Antibody
SKU:
CAB19705
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2237
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Predicted to enable ATP binding activity. Involved in chaperone-mediated protein complex assembly and protein insertion into mitochondrial outer membrane. Located in cytosol and extracellular exosome. Implicated in Chagas disease. Biomarker of chronic obstructive pulmonary disease; rheumatoid arthritis; type 2 diabetes mellitus; and ulcerative colitis.
Purification Method
Affinity purification
Gene ID
3308
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSPA4 Rabbit mAb (CAB19705) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HSPA4 Rabbit mAb (CAB19705) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using HSPA4 Rabbit mAb (CAB19705) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
