Description
Anti-Human CD119 (IFN-γ Rα Chain) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD119 (IFN-γ Rα Chain) In Vivo Antibody - Low Endotoxin from Assay Genie, a meticulously crafted monoclonal antibody tailored for in vivo applications. This antibody specifically targets the CD119 protein (also known as the interferon-gamma receptor alpha chain), which plays a vital role in immune signaling and response. This makes it an exceptional choice for research in immunology, particularly in studies involving interferon signaling and immune modulation. Possessing a high-purity preparation and low endotoxin levels (<1.0 EU/mg), this antibody is perfect for use in ELISA, flow cytometry, immunohistochemistry, and various other assays.
It is available in multiple sizes and is formulated in phosphate-buffered saline to ensure both stability and efficacy. Enhance your research with this reliable and versatile antibody, designed to provide consistent and reproducible results. CD119 is a critical component of the IFN-γ receptor complex, primarily expressed on the surface of immune cells. It is essential for mediating the biological effects of interferon-gamma, influencing processes such as antigen presentation, macrophage activation, and the regulation of adaptive immunity.
| Product Name: | Anti-Human CD119 (IFN-γ Rα Chain) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0103 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | GIR-208 |
| Protein: | IFN-gamma R-alpha |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IFNGR1, IFNg Ra |
| Isotype: | Mouse IgG1 |
| Reactivity: | Human |
| Immunogen: | Human IFN-gamma R-alpha, Purified from human placenta |
| Applications: | FA, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC |
| Recommended Usage: | FC The suggested concentration for clone GIR-208 purified antibody for staining cells in flow cytometry is ≤ 0.5 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone GIR-208 recognizes an epitope on human IFN-gamma R-alpha. |
| Antigen Distribution: | The IFN-gamma receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. |
| Immunogen: | Human IFN-gamma R-alpha, Purified from human placenta |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and β chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription.
| Technical Datasheet: | View |
| Protein: | IFN-gamma R-alpha |
| Research Area: | Immunology |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
